Magnetic alginate microparticles for purification of .alpha.-amylases

0170718 - UEK-B 20033023 RIV NL eng J - Článek v odborném periodiku
Šafaříková, Miroslava - Roy, I. - Gupta, M. N. - Šafařík, Ivo
Magnetic alginate microparticles for purification of .alpha.-amylases.
Journal of Biotechnology. Roč. 105, - (2003), s. 255-260. ISSN 0168-1656. E-ISSN 1873-4863
Grant CEP: GA MŠMT OC 523.80; GA AV ČR IBS6087204
Výzkumný záměr: CEZ:AV0Z6087904
Klíčová slova: alginate * ferrofluid * amalyses
Kód oboru RIV: CE - Biochemie
Impakt faktor: 2.543, rok: 2003

Spherical magnetic alginate microparticles (25 - 60 .micro.m in diameter) were prepared using the microemulsion system, with water saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solutions. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of .alpha.-amylases. Enzyme activity was eluted by 1.0 M maltose. Alpha-amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96 % activity recovery, respectively.
Trvalý link: http://hdl.handle.net/11104/0067800