Neo-sex chromosomes in the monarch butterfly, Danaus plexippus

0479435 - BC 2018 RIV US eng J - Článek v odborném periodiku
Mongue, A. J. - Nguyen, Petr - Voleníková, Anna - Walters, J. R.
Neo-sex chromosomes in the monarch butterfly, Danaus plexippus.
G3-Genes, Genomes, Genetics. Roč. 7, č. 10 (2017), s. 3281-3294. ISSN 2160-1836. E-ISSN 2160-1836
Grant CEP: GA ČR(CZ) GA14-22765S; GA ČR(CZ) GP14-35819P
Grant ostatní: GA JU(CZ) 159/2016/P
Institucionální podpora: RVO:60077344
Klíčová slova: sex chromosomes * evolution * Lepidoptera
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 2.742, rok: 2017
http://www.g3journal.org/content/7/10/3281.long

We report the discovery of a neo-sex chromosome in the monarch butterfly, Danaus plexippus, and several of its close relatives. Z-linked scaffolds in the D. plexippus genome assembly were identified via sex-specific differences in Illumina sequencing coverage. Additionally, a majority of the D. plexippus genome assembly was assigned to chromosomes based on counts of one-to-one orthologs relative to the butterfly Melitaea cinxia (with replication using two other lepidopteran species), in which genome scaffolds have been mapped to linkage groups. Sequencing coverage-based assessments of Z linkage combined with homology-based chromosomal assignments provided strong evidence for a Z-autosome fusion in the Danaus lineage, involving the autosome homologous to chromosome 21 in M. cinxia. Coverage analysis also identified three notable assembly errors resulting in chimeric Z-autosome scaffolds. Cytogenetic analysis further revealed a large W chromosome that is partially euchromatic, consistent with being a neo-W chromosome. The discovery of a neo-Z and the provisional assignment of chromosome linkage for >90% of D. plexippus genes lays the foundation for novel insights concerning sex chromosome evolution in this female-heterogametic model species for functional and evolutionary genomics.
Trvalý link: http://hdl.handle.net/11104/0276084