Increased Binding of Calcium Ions at Positively Curved Phospholipid Membranes

0474257 - ÚOCHB 2018 RIV US eng J - Článek v odborném periodiku
Magarkar, Aniket - Jurkiewicz, Piotr - Allolio, Christoph - Hof, Martin - Jungwirth, Pavel
Increased Binding of Calcium Ions at Positively Curved Phospholipid Membranes.
Journal of Physical Chemistry Letters. Roč. 8, č. 2 (2017), s. 518-523. ISSN 1948-7185
Grant CEP: GA ČR(CZ) GA16-01074S; GA ČR(CZ) GAP207/12/0919
Grant ostatní: AV ČR(CZ) AP1102
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:61388963 ; RVO:61388955
Klíčová slova: molecular dynamics * fluorescence spectroscopy * calcium * phospholipids
Obor OECD: Physical chemistry; Physical chemistry (UFCH-W)
Impakt faktor: 8.709, rok: 2017

Calcium ion is the ubiquitous messenger in cells and plays a key role in neuronal signaling and fusion of synaptic vesicles. These vesicles are typically similar to 20-50 nm in diameter, and thus their interaction with calcium ions cannot be modeled faithfully with a conventional flat membrane bilayer setup. Within our newly developed molecular dynamics simulations setup, we characterize here interactions of the calcium ion with curved membrane interfaces with atomistic detail. The present molecular dynamics simulations together with time-dependent fluorescence shift experiments suggest that the mode and strength of interaction of calcium ion with a phospholipid bilayer depends on its curvature. Potential of mean force calculations demonstrate that the binding of calcium ion to the positively curved side of the bilayer is significantly stronger compared with that to a flat membrane.
Trvalý link: http://hdl.handle.net/11104/0271384