Reductively degradable poly(2-hydroxyethyl methacrylate) hydrogels with oriented porosity for tissue engineering applications

0473652 - ÚMCH 2018 RIV US eng J - Článek v odborném periodiku
Macková, Hana - Plichta, Zdeněk - Hlídková, Helena - Sedláček, Ondřej - Konefal, Rafal - Sadakbayeva, Zhansaya - Dušková-Smrčková, Miroslava - Horák, Daniel - Kubinová, Šárka
Reductively degradable poly(2-hydroxyethyl methacrylate) hydrogels with oriented porosity for tissue engineering applications.
ACS Applied Materials and Interfaces. Roč. 9, č. 12 (2017), s. 10544-10553. ISSN 1944-8244. E-ISSN 1944-8252
Grant CEP: GA ČR(CZ) GA14-14961S
Institucionální podpora: RVO:61389013 ; RVO:68378041
Klíčová slova: poly(2-hydroxyethyl methacrylate) * 2-methacryloyloxyethyl phosphorylcholine * hydrogel
Obor OECD: Polymer science; Nano-materials (production and properties) (UEM-P)
Impakt faktor: 8.097, rok: 2017

Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (Mw = 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2´-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 mu m and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in alpha-minimum essential medium, respectively.
Trvalý link: http://hdl.handle.net/11104/0270986