Recent developments and applications of capillary and microchip electrophoresis in proteomic and peptidomic analyses

0458688 - ÚOCHB 2017 RIV DE eng J - Článek v odborném periodiku
Štěpánová, Sille - Kašička, Václav
Recent developments and applications of capillary and microchip electrophoresis in proteomic and peptidomic analyses.
Journal of Separation Science. Roč. 39, č. 1 (2016), s. 198-211. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA ČR(CZ) GA13-17224S; GA ČR(CZ) GA15-01948S
Institucionální podpora: RVO:61388963
Klíčová slova: capillary electrophoresis * mass spectrometry * microchip electrophoresis * peptidomics * proteomics
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.557, rok: 2016

This review presents recent developments and applications of capillary and microchip electromigration methods in proteomics and peptidomics. Sample preparation methods as well as instrumental innovations in the coupling of these advanced electromigration methods with mass spectrometry detection employed in proteomic and peptidomic analyses are presented. Interesting applications of various capillary electromigration methods in bottom-up as well as top-down proteomics, including investigation of post-translational modifications of proteins are described. In addition, several examples of the use of capillary electromigration methods combined with mass spectrometry detection in clinical proteomics and peptidomics are demonstrated.
Trvalý link: http://hdl.handle.net/11104/0258941