Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems

0452625 - MBÚ 2016 RIV GB eng J - Článek v odborném periodiku
Mathis, A. - Depaquit, J. - Dvořák, V. - Tuten, H. - Banuls, A.-L. - Halada, Petr - Zapata, S. - Lehrter, V. - Hlaváčková, K. - Prudhomme, J. - Volf, P. - Sereno, D. - Kaufmann, Ch. - Pflueger, V. - Schaffner, F.
Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems.
Parasites & Vectors. Roč. 8, MAY 2015 (2015). ISSN 1756-3305. E-ISSN 1756-3305
Grant CEP: GA ČR(CZ) GA15-04329S
Institucionální podpora: RVO:61388971
Klíčová slova: Arthropod identification * Bruker * Centralized reference database
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.234, rok: 2015

Identical biomarker mass patterns ('superspectra') were obtained with colony-or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 degrees C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87 % (140/161); specificity was 100 %. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3 %; the specificity was 100 %. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7 % and 97.4 %, respectively.
Trvalý link: http://hdl.handle.net/11104/0253575