Study of Streptavidin-Modified Quantum Dots by Capillary Electrophoresis

0421227 - ÚFM 2014 RIV DE eng J - Článek v odborném periodiku
Stanisavljevic, M. - Janů, L. - Šmerková, K. - Křížková, S. - Pizúrová, Naděžda - Ryvolová, M. - Adam, V. - Hubálek, J. - Kizek, R.
Study of Streptavidin-Modified Quantum Dots by Capillary Electrophoresis.
Chromatographia. Roč. 76, 7-8 (2013), s. 335-343. ISSN 0009-5893. E-ISSN 1612-1112
Institucionální podpora: RVO:68081723
Klíčová slova: Capillary electrophoresis * Gel electrophoresis * Avidin-biotin technology * Oligonucleotide * Nanoparticle * quantum dots
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.370, rok: 2013

Great boom of nanotechnologies impacts almost all areas of science and therefore detail understanding of the properties of nanomaterials as well as their interaction abilities is required. Surface modification and functionalization of nanoparticles is of a great interest due to the wide range of applications in the area of nanomedicine, nanobiology, and/or biochemistry. In this study, CdTe QDs were synthesized using microwave reactor and their surface was modified by streptavidin to ensure further suitability for bioconjugation with biotin-labelled oligonucleotides. For characterization of the synthesized QDs and for monitoring of the interaction with the oligonucleotide, capillary and gel electrophoresis was used.
Trvalý link: http://hdl.handle.net/11104/0229728