Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina

0420954 - BC 2014 RIV GB eng J - Článek v odborném periodiku
Hernández-Orts, Jesús S. - Aznar, F. J. - Blasco-Costa, Maria Isabel - García, N.A. - Víllora-Montero, M. - Crespo, E.A. - Raga, J. A. - Montero, F. E.
Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina.
Parasites & Vectors. Roč. 6, AUG 2013 (2013), s. 252. ISSN 1756-3305. E-ISSN 1756-3305
Institucionální podpora: RVO:60077344
Klíčová slova: Anisakidae * Eealworms * Pseudoterranova cattani * taxonomy * Ecology * Cox1 * Marine fishes * Southwestern Atlantic
Kód oboru RIV: EH - Ekologie - společenstva
Impakt faktor: 3.251, rok: 2013

Methods: A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results: A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver.
Trvalý link: http://hdl.handle.net/11104/0227479