Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles

0396901 - FGÚ 2014 RIV CZ eng J - Článek v odborném periodiku
Žurmanová, J. - Soukup, Tomáš
Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles.
Physiological Research. Roč. 62, č. 4 (2013), s. 445-453. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA ČR(CZ) GA304/08/0256; GA ČR(CZ) GA305/09/1228; GA MŠMT(CZ) 7AMB12SK158; GA MŠMT(CZ) LH12058
Grant ostatní: EC(XE) LSH-CT-2004-511978
Výzkumný záměr: CEZ:AV0Z50110509
Institucionální podpora: RVO:67985823
Klíčová slova: rat * soleus * extensor digitorum longus * myosin heavy chain isoforms * muscle gene expression * quantitative Real Time RT-PCR * SDS-PAGE * fiber type analysis
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 1.487, rok: 2013

We studied the expression of myosin heavy chain isoforms at mRNA and protein levels as well as fiber type composition in the fast extensor digitorum longus (EDL) and slow soleus (SOL) twitch muscles of adult inbred Lewis strain rats. Based on our data presenting quantitative evidence of corresponding proportions between mRNA level, protein content and fiber type composition, we suggest that the Real Time RT-PCR technique can be used as a routine method for analysis of muscle composition changes and could be advantageous for the analysis of scant biological samples such as muscle biopsies in humans
Trvalý link: http://hdl.handle.net/11104/0224569