Superhelical DNA as a preferential binding target of 14-3-3 gamma protein

0389526 - BFÚ 2013 US eng J - Článek v odborném periodiku
Brázda, Václav - Čechová, J. - Coufal, Jan - Rumpel, S. - Jagelská, Eva
Superhelical DNA as a preferential binding target of 14-3-3 gamma protein.
[Superhelical DNA as a preferential binding target of 14-3-3 gamma protein.]
Journal of Biomolecular Structure & Dynamics. Roč. 30, č. 4 (2012), s. 371-378. ISSN 0739-1102
Grant CEP: GA ČR(CZ) 301/10/1211; GA MŠMT(CZ) LC 06035; GA AV ČR(CZ) M200040904
Institucionální podpora: RVO:68081707
Klíčová slova: TUMOR-SUPPRESSOR P53 * ASSOCIATES IN-VIVO * SUPERCOILED DNA
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.986, rok: 2010

The 14-3-3 protein family is a highly conserved and widely distributed group of proteins consisting of multiple isoforms in eukaryotes. Ubiquitously expressed, 14-3-3 proteins play key roles in DNA replication, cell cycle regulation, and apoptosis. The function of 14-3-3 proteins is mediated by interaction with a large number of other proteins and with DNA. It has been demonstrated that 14-3-3 gamma protein binds strongly to cruciform structures and is crucial for initiating replication. In this study, we analyzed DNA binding properties of the 14-3-3 gamma isoform to linear and supercoiled DNA.

The 14-3-3 protein family is a highly conserved and widely distributed group of proteins consisting of multiple isoforms in eukaryotes. Ubiquitously expressed, 14-3-3 proteins play key roles in DNA replication, cell cycle regulation, and apoptosis. The function of 14-3-3 proteins is mediated by interaction with a large number of other proteins and with DNA. It has been demonstrated that 14-3-3 gamma protein binds strongly to cruciform structures and is crucial for initiating replication. In this study, we analyzed DNA binding properties of the 14-3-3 gamma isoform to linear and supercoiled DNA.
Trvalý link: http://hdl.handle.net/11104/0218405