A 3,000-Loci Transcription Map of Chromosome 3B Unravels the Structural and Functional Features of Gene Islands in Hexaploid Wheat

0374549 - ÚEB 2012 RIV US eng J - Článek v odborném periodiku
Rustenholz, C. - Choulet, F. - Laugier, Ch. - Šafář, Jan - Šimková, Hana - Doležel, Jaroslav - Magni, F. - Scalabrin, S. - Cattonaro, F. - Vautrin, S. - Bellec, A. - Berges, H. - Feuillet, C. - Paux, E.
A 3,000-Loci Transcription Map of Chromosome 3B Unravels the Structural and Functional Features of Gene Islands in Hexaploid Wheat.
Plant Physiology. Roč. 157, č. 4 (2011), s. 1596-1608. ISSN 0032-0889. E-ISSN 1532-2548
Grant CEP: GA MŠMT ED0007/01/01
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: LOCAL COEXPRESSION DOMAINS * ARABIDOPSIS-THALIANA * NEIGHBORING GENES
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 6.535, rok: 2011

To improve our understanding of the organization and regulation of the wheat (Triticum aestivum) gene space, we established a transcription map of a wheat chromosome (3B) by hybridizing a newly developed wheat expression microarray with bacterial artificial chromosome pools from a new version of the 3B physical map as well as with cDNA probes derived from 15 RNA samples. Mapping data for almost 3,000 genes showed that the gene space spans the whole chromosome 3B with a 2-fold increase of gene density toward the telomeres due to an increase in the number of genes in islands. Comparative analyses with rice (Oryza sativa) and Brachypodium distachyon revealed that these gene islands are composed mainly of genes likely originating from interchromosomal gene duplications. Gene Ontology and expression profile analyses for the 3,000 genes located along the chromosome revealed that the gene islands are enriched significantly in genes sharing the same function or expression profile.
Trvalý link: http://hdl.handle.net/11104/0207446