Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays

0370713 - BFÚ 2012 RIV GB eng J - Článek v odborném periodiku
Horáková Brázdilová, Petra - Macíčková-Cahová, Hana - Pivoňková, Hana - Špaček, Jan - Havran, Luděk - Hocek, Michal - Fojta, Miroslav
Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays.
Organic & Biomolecular Chemistry. Roč. 9, č. 5 (2011), s. 1366-1371. ISSN 1477-0520. E-ISSN 1477-0539
Grant CEP: GA MŠMT(CZ) LC06035; GA MŠMT(CZ) LC512; GA AV ČR(CZ) IAA400040901
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702; CEZ:AV0Z40550506
Klíčová slova: DNA tail-labelling * protein-DNA binding * DNA hybridization
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.696, rok: 2011

A simple approach to DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates is presented. We demonstrated a perfect discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes as well as the ability of tumour suppressor p53 protein to recognize a specific binding site within tail-labelled DNA.
Trvalý link: http://hdl.handle.net/11104/0204428