Characterization of the capillary network in skeletal muscles from 3D data

0359123 - FGÚ 2012 RIV CZ eng J - Článek v odborném periodiku
Eržen, I. - Janáček, Jiří - Kubínová, Lucie
Characterization of the capillary network in skeletal muscles from 3D data.
Physiological Research. Roč. 60, č. 1 (2011), s. 1-13. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA MŠMT(CZ) MEB090910; GA MŠMT(CZ) LC06063
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: capillaries * skeletal muscle * confocal microscopy * stereology * 3D analysis * 3D visualization
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.555, rok: 2011

Immunohistochemical methods for visualization of capillaries and muscle fibres in thick muscle sections are presented. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area or fibre volume are evaluated and 3D models of capillaries and muscle fibres are produced. The described 3D methodology is applied to the anatomical remodelling of capillarity during acute denervation and early reinnervation in the rat soleus and extensor digitorum longus muscles
Trvalý link: http://hdl.handle.net/11104/0196967