Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma

0354940 - ÚMG 2012 RIV DE eng J - Článek v odborném periodiku
Chan, K.C. - Ko, J.M. - Lung, H.L. - Sedláček, Radislav - Zhang, Z.F. - Luo, D.Z. - Feng, Z.B. - Chen, S. - Chen, H. - Chan, K.W. - Tsao, S.W. - Chua, D.T. - Zabarovsky, E.R. - Stanbridge, E.J. - Lung, M.L.
Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma.
International Journal of Cancer. Roč. 129, č. 8 (2011), s. 1826-1837. ISSN 0020-7136. E-ISSN 1097-0215
Grant ostatní: Research Grants Council of the Hong Kong Special Administrative Region(CN) HKU661708M
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: MMP19 * nasopharyngeal carcinoma * tumor suppressor gene * angiogenesis
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.444, rok: 2011

The association of matrix metalloproteinase-19 (MMP19) in the development of nasopharyngeal carcinoma (NPC) was identified from differential gene profiling, which showed MMP19 was one of the candidate genes down-regulated in the NPC cell lines. In this study, analysis showed MMP19 was down-regulated in all seven NPC cell lines and in 69.7% of primary NPC specimens. Allelic deletion and promoter hypermethylation contribute to MMP19 down-regulation. In the in vivo tumorigenicity assay, only the wild-type, but not mutant, MMP19 transfectants suppress tumor formation in nude mice. In the in vitro colony formation assay, WT MMP19 dramatically reduces colony-forming ability of NPC cell lines. In the tube formation assay secreted WT MMP19, but not mutant MMP19, induces reduction of tube-forming ability in endothelial cells with decreased vascular endothelial growth factor. The anti-angiogenic activity of WT MMP19 is correlated with suppression of tumor formation.
Trvalý link: http://hdl.handle.net/11104/0193830