Bioluminescent Bioreporter Pseudomonas putida TVA8 as a Detector of Water Pollution. Operational Conditions and Selectivity of Free Cells Sensor

0354863 - ÚCHP 2011 RIV NL eng J - Článek v odborném periodiku
Kuncová, Gabriela - Pazlarová, J. - Hlavatá, Alena - Ripp, S. - Sayler, G.S.
Bioluminescent Bioreporter Pseudomonas putida TVA8 as a Detector of Water Pollution. Operational Conditions and Selectivity of Free Cells Sensor.
Ecological Indicators. Roč. 11, č. 3 (2011), s. 882-887. ISSN 1470-160X. E-ISSN 1872-7034
Grant CEP: GA MŠMT ME 893
Výzkumný záměr: CEZ:AV0Z40720504
Klíčová slova: whole-cell biosensor * bioluminiscence * pseudomonas putida TVA8
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 2.695, rok: 2011

Bioluminescence induction was tested in minimal medium. At 15 °C the highest bioluminescence induced with toluene (1.325 mg/L) was reached after 6 h. At 25 °C the bioluminescence maximum was approximately 20% lower but this was reached after 3.5 h, and at temperatures of 7 °C, 28 °C, 30 °C and 34 °C, bioluminescence peaked at ≤60% of the maximum. Time courses of bioluminescence were dependent on cell concentrations. The heights of bioluminescence maxima were proportional to toluene concentration in the range 0–26 mg/L. Twenty-three organic pollutants were tested as bioluminescent inducers. Bioluminescence was also induced with ethanol and methanol and the presence of these alcohols in concentrations of ≤1% increased bioluminescence of toluene. The induction of bioluminescence from samples of wastewater and groundwater contaminated with BTEX from 0.5 to 120 mg/L was demonstrated.
Trvalý link: http://hdl.handle.net/11104/0193771