Development of soil microbiology methods: from respirometry to molecular approaches

0354462 - MBÚ 2011 RIV DE eng J - Článek v odborném periodiku
Gabriel, Jiří
Development of soil microbiology methods: from respirometry to molecular approaches.
Journal of Industrial Microbiology & Biotechnology. Roč. 37, č. 12 (2010), s. 1289-1297. ISSN 1367-5435. E-ISSN 1476-5535
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Soil microbiology * Continuous cultivation * Microbial growth
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.416, rok: 2010

This review deals with techniques and methods used in the study of the function and development of microorganisms occurring in soil with emphasis on the contributions of Czech Academician Ivan Malek and his coworkers or fellows (JiA (TM) i Macura, Frantiek Kunc) to the development of basic techniques used in soil microbiology. Early studies, including batch cultivation and respirometric techniques, as well as later developments of percolation and continuous-flow methods of cultivation of soil microorganisms are discussed. Recent developments in the application of analytical chemistry (HPLC or GC) and of molecular biological techniques to ecological questions that have revolutionized concepts in soil microbiology and microbial ecology are also briefly mentioned, including denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), phospholipid fatty acid analysis (PLFA) and others
Trvalý link: http://hdl.handle.net/11104/0193458