Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process

0349615 - ÚIACH 2011 RIV GB eng J - Článek v odborném periodiku
Laštovičková, Markéta - Mazanec, Karel - Smětalová, Dagmar - Bobálová, Janette
Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process.
Journal of the Institute of Brewing. Roč. 116, č. 3 (2010), s. 245-250. ISSN 0046-9750. E-ISSN 2050-0416
Grant CEP: GA MŠMT 1M0570
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: barley * brewing * glycation
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 0.897, rok: 2010

The analysis of intact proteins, extracted from the barley grain, the malt and the aliquots collected during malting and presented in this paper, show the feasibility of using the linear mode of MALDI-TOF MS to obtain a profile of the proteins present at all stages of the malting process. The best and the most reproducible results were obtained after a desalting procedure using Nanosep 3K and applying DHAP as a matrix for the MS measurement of the intact proteins.
Trvalý link: http://hdl.handle.net/11104/0189805