Biochemical and Genetic Insights into Asukamycin Biosynthesis

0348161 - MBÚ 2011 RIV US eng J - Článek v odborném periodiku
Rui, Z. - Petříčková, Kateřina - Škanta, František - Pospíšil, Stanislav - Yang, Y. - Chen, Ch.-Y. - Tsai, S.-F. - Floss, H. G. - Petříček, Miroslav - Yu, T.-W.
Biochemical and Genetic Insights into Asukamycin Biosynthesis.
Journal of Biological Chemistry. Roč. 285, č. 32 (2010), s. 24915-24924. ISSN 0021-9258. E-ISSN 1083-351X
Grant CEP: GA MŠMT 2B06154
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: NODOSUS SUBSP ASUKAENSIS * FATTY-ACID SYNTHASE * CRYSTAL-STRUCTURE
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 5.328, rok: 2010

Asukamycin, a member of the manumycin family metabolites, is an antimicrobial and potential antitumor agent isolated from Streptomyces nodosus subsp. asukaensis. The entire asukamycin biosynthetic gene cluster was cloned, assembled, and expressed heterologously in Streptomyces lividans. Bioinformatic analysis and mutagenesis studies elucidated the biosynthetic pathway at the genetic and biochemical level. Four gene sets, asuA–D, govern the formation and assembly of the asukamycin building blocks: a 3-amino-4-hydroxybenzoic acid core component, a cyclohexane ring, two triene polyketide chains, and a 2-amino-3-hydroxycyclopent-2-enone moiety to form the intermediate protoasukamycin
Trvalý link: http://hdl.handle.net/11104/0188761