Chemical conjugation of cowpea mosaic viruses with reactive HPMA-based polymers

0347069 - ÚMCH 2011 RIV NL eng J - Článek v odborném periodiku
Laga, Richard - Koňák, Čestmír - Šubr, Vladimír - Ulbrich, Karel - Suthiwangcharoen, N. - Niu, Q. - Wang, Q.
Chemical conjugation of cowpea mosaic viruses with reactive HPMA-based polymers.
Journal of Biomaterial Science Polymer Edition. Roč. 21, č. 12 (2010), s. 1669-1685. ISSN 0920-5063. E-ISSN 1568-5624
Grant CEP: GA AV ČR KJB400500803; GA ČR GA202/09/2078; GA AV ČR KAN200200651
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: acylthiazolidine-2-thione reactive groups * bioconjugation * coating
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 1.842, rok: 2010

N-(2-Hydroxypropyl)methacrylamide (HPMA) co-polymers containing disulfide and carbonyl thiazolidine-2-thione (TT) reactive groups in their side-chains (pHPMA-TT) were used as reductively removable chemical modification of the surface of cowpea mosaic viruses (CPMV). CPMV was used in this study as a model particle for viral gene delivery. The co-polymer reaction with CPMV surfaces carried out in aqueous solution was evaluated by monitoring the changes in the weight-average molecular weight and hydrodynamic size of the viruses using light scattering methods.
Trvalý link: http://hdl.handle.net/11104/0006004