Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines

0346582 - BFÚ 2011 RIV US eng J - Článek v odborném periodiku
Pivoňková, Hana - Horáková Brázdilová, Petra - Fojtová, Miloslava - Fojta, Miroslav
Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines.
Analytical Chemistry. Roč. 82, č. 16 (2010), s. 6807-6813. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA AV ČR(CZ) IAA400040901; GA MŠMT(CZ) LC06035
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: modified DNA * 7-deazapurines * voltammetric analysis
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 5.874, rok: 2010

Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases. We show that enzymatic incorporation of G* can easily be monitored by simple ex situ voltammetric analysis of the modified DNA at carbon electrodes. G* is shown as an attractive electroactive marker for DNA, efficiently incorporable by PCR.
Trvalý link: http://hdl.handle.net/11104/0187576