AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis

0337486 - ÚEB 2010 RIV US eng J - Článek v odborném periodiku
Zhang, J. - Vaňková, Radomíra - Malbeck, Jiří - Dobrev, Petre - Xu, Y. - Chong, K. - Neff, M. M.
AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis.
PLoS ONE. Roč. 4, č. 12 (2009), Art_no_-e8236. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR GA206/06/1306
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: TRANSGENIC TOBACCO PLANTS * OXIDASE/DEHYDROGENASE GENES * ISOPENTENYL TRANSFERASE
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 4.351, rok: 2009

Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(∆2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.
Trvalý link: http://hdl.handle.net/11104/0181466