GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column

0335962 - BC 2010 RIV DE eng J - Článek v odborném periodiku
Zahradníčková, Helena - Hušek, Petr - Šimek, Petr
GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column.
Journal of Separation Science. Roč. 32, č. 22 (2009), s. 3919-3924. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA ČR GA203/09/2014
Výzkumný záměr: CEZ:AV0Z50070508
Klíčová slova: chiral separation * derivatization * D,L-AAs
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.551, rok: 2009

Heptafluorobutyl chloroformate, a recently introduced derivatization reagent, was examined in enantioseparation of amino acids by gas chromatography. Twenty proteinogenic amino acids, plus ornithine, cystine and 4-fluorophenylalanine (internal standard) were treated with the reagent and separation properties of the derivatives assessed on a Chirasil-Val capillary column. Nineteen amino acid enantiomers were efficiently separated in 43 minutes except proline, arginine and cystine. The heptafluorobutyl chloroformate derivatives of the studied DL-amino acids show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediate one-step reaction-extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of heptafluorobutyl chloroformate to chiral amino acid analysis.
Trvalý link: http://hdl.handle.net/11104/0180302