A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples

0084909 - ÚBO 2008 RIV PL eng J - Článek v odborném periodiku
Kaňuch, Peter - Hájková, Petra - Řehák, Z. - Bryja, Josef
A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples.
[Rychlý PCR-test na druhovou identifikaci dvou kryptických druhů netopýrů Pipistrellus pipistrellus a P. pygmaeus a jeho aplikace na muzejní vzorky a vzorky trusu.]
Acta Chiropterologica. Roč. 9, č. 1 (2007), s. 277-282. ISSN 1508-1109. E-ISSN 1733-5329
Grant CEP: GA ČR GA206/06/0954
Výzkumný záměr: CEZ:AV0Z60930519
Klíčová slova: bats * cytochrome b * mtDNA * sibling species * non-invasive sampling
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.857, rok: 2007
http://www.ingentaconnect.com/content/miiz/actac/2007/00000009/00000001/art00022

The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.

V práci byl optimalizován rychlý test založený na metodě PCR, který produkuje PCR produkty specifické pro jednotlivé druhy Pipistrellus pygmaeus a P. pipistrellus. Test je možno rovněž použít na muzejní materiál a na vzorky DNA z trusu.
Trvalý link: http://hdl.handle.net/11104/0147534