Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant

0369355 - BC 2012 RIV US eng J - Článek v odborném periodiku
Charles, J.-P. - Iwema, T. - Epa, V. C. - Takaki, Keiko - Ryneš, J. - Jindra, Marek
Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 108, č. 52 (2011), s. 21128-21133. ISSN 0027-8424. E-ISSN 1091-6490
Grant CEP: GA ČR(CZ) GD204/09/H058; GA ČR(CZ) GA204/07/1032; GA AV ČR IAA500960906
GRANT EU: European Commission(XE) 276569 - JHRECEPTOR
Výzkumný záměr: CEZ:AV0Z50070508
Klíčová slova: structure modeling * insecticide action * metamorphosis
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 9.681, rok: 2011

Juvenile hormone (JH) is a terpenoid, a small lipophilic molecule of vital importance to insects. The molecular action of JH has for long decades remained an enigma, because a JH receptor could not be identified. In this study we provide conclusive evidence for the JH receptor role of the bHLH-PAS domain transcription factor Methoprene-tolerant (Met). By testing Met point mutant proteins in vitro, we determined specific amino acids contributing to the high-affinity JH binding. Using mutants unable to bind JH, we demonstrated that interactions of Met with partner proteins require the ligand-binding capacity of Met. Therefore, Met resembles certain nuclear receptors, e. g., of retinoic acid or thyroid hormone, but since Met belongs to a different protein family, it establishes a prototype of a hormonal receptor of a novel class. Our results address a long-standing question of the mechanism of action of juvenile hormone and its insecticidal analogs.
Trvalý link: http://hdl.handle.net/11104/0203440