Polyphasic characterization of Dolichospermum spp. and Sphaerospermopsis spp. (Nostocales, cyanobacteria): morphology, 16S rRNA gene sequences and fatty acid and secondary metabolite profiles

0366574 - BC 2012 RIV US eng J - Článek v odborném periodiku
Zapomělová, Eliška - Hrouzek, Pavel - Řezanka, Tomáš - Jezberová, Jitka - Řeháková, Klára - Hisem, D. - Komárková, Jaroslava
Polyphasic characterization of Dolichospermum spp. and Sphaerospermopsis spp. (Nostocales, cyanobacteria): morphology, 16S rRNA gene sequences and fatty acid and secondary metabolite profiles.
Journal of Phycology. Roč. 47, č. 5 (2011), s. 1152-1163. ISSN 0022-3646. E-ISSN 1529-8817
Grant CEP: GA AV ČR(CZ) KJB600960703; GA ČR(CZ) GAP504/10/1501; GA ČR(CZ) GA206/09/0309
Výzkumný záměr: CEZ:AV0Z60170517; CEZ:AV0Z50200510; CEZ:AV0Z60050516
Klíčová slova: taxonomy * cyanobacteria * Anabaena * Dolichospermum * Sphaerospermopsis * phylogeny * 16S rRNA gene * fatty acids * secondary metabolites
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.071, rok: 2011

The genera Dolichospermum and Sphaerospermopsis represent a highly diversified group of planktonic cyanobacteria that have been recently separated from the traditional genus Anabaena. In this study, morphological diversity, phylogeny of the 16S rRNA gene, production of fatty acids, and secondary metabolite profiles were evaluated in 33 strains of 14 morphospecies isolated from the Czech Republic. Clustering of the strains based on 16S rRNA gene sequences corresponded to wider groups of species in terms of morphology. The overall secondary metabolite and fatty acid profiles, however, were not correlated to each other and neither were they correlated to the 16S rRNA phylogeny nor the morphology of the strains. Nevertheless, a minor part of the detected secondary metabolites (19% of all compounds) was present only in close relatives and can be thus considered as autapomorphic features.
Trvalý link: http://hdl.handle.net/11104/0201518