The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography

0364545 - ÚŽFG 2012 RIV NL eng J - Článek v odborném periodiku
Czauderna, M. - Kowalczyk, J. - Marounek, Milan
The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 879, č. 23 (2011), s. 2251-2258. ISSN 1570-0232. E-ISSN 1873-376X
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: Malondialdehyde * 2,4-Dinitrophenylhydrazine * High performance liquid chromatography
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.888, rok: 2011

A method for the determination of malondialdehyde (MDA) concentrations in specimens of animal tissues and feed has been developed using high performance liquid chromatography. The MDA concentration in acidified urine samples was determined after its conversion with 2,4-dinitrophenylhydrazine (DNPH) to a hydrazone (MDA-DNPH). Samples of blood plasma, muscle, liver and feed were prepared by saponification followed by derivatisation with DNPH to MDA-DNPH. The MDA concentration in chicken and hen feed samples was analysed after saponification and derivatisation followed by extractions with hexane. The free MDA in plasma samples was determined after deproteinization followed by derivatisation of MDA with DNPH. The chromatographic separation of MDA-DNPH samples was conducted using Phenomenex C(18)-columns (Synergi 2.5 mu m, Hydro-RP, 100 angstrom, the length of 100 mm) with an inner diameter of 2 or 3 mm.
Trvalý link: http://hdl.handle.net/11104/0200008