Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi

0359409 - BC 2012 RIV NL eng J - Článek v odborném periodiku
Paris, Zdeněk - Hashimi, Hassan - Lun, Sijia - Alfonzo, J. D. - Lukeš, Julius
Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi.
Molecular and Biochemical Parasitology. Roč. 176, č. 2 (2011), 116-120. ISSN 0166-6851. E-ISSN 1872-9428
Grant CEP: GA ČR GA204/09/1667; GA MŠMT LC07032
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: Trypanosoma * tRNA * Protein import * Mitochondrion * Kinetoplast
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.551, rok: 2011

Trypanosoma brucei brucei has two distinct developmental stages, the procyclic stage in the insect and the bloodstream stage in the mammalian host. The significance of each developmental stage is punctuated by specific changes in metabolism. In the insect, T. b. brucei is strictly dependent on mitochondrial function and thus respiration to generate the bulk of its ATP, whereas in the mammalian host it relies heavily on glycolysis. Peculiarly, akinetoplastic strains of T. b. evansi that lack mitochondrial DNA do exist in the wild and are developmentally locked in the glycolysis-dependent bloodstream stage. Using RNAi we show that two mitochondrion-imported proteins, mitochondrial RNA polymerase and guide RNA associated protein 1, are still imported into the nucleic acids-lacking organelle of T. b. evansi, making the need for these proteins futile.
Trvalý link: http://hdl.handle.net/11104/0197205