Single-step electrospinning to bioactive polymer nanofibers

0358513 - ÚMCH 2012 RIV US eng J - Článek v odborném periodiku
Gentsch, R. - Pippig, F. - Schmidt, S. - Černoch, Peter - Polleux, J. - Börner, H. G.
Single-step electrospinning to bioactive polymer nanofibers.
Macromolecules. Roč. 44, č. 3 (2011), s. 453-461. ISSN 0024-9297. E-ISSN 1520-5835
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: electrospinning * polymer-peptide conjugate * block copolymer
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 5.167, rok: 2011

A single-step process to get polymer nanofiber meshes of biofunctional peptide segments is described in the article. A standard electrospinning of a homogeneous mixture of a polymer-peptide conjugate (poly(lactic acid)-block-CGGRGDS) and a biocompatible commodity poly(lactic-co-glycolic acid) (PLGA) leads to nonwovens with the peptide part enriched on the fiber surface. The accessibility of the peptide is proved by contact angle measurements as well as by probing electrostatic interaction between CGGRGDS surface functionalities and a colloidal silica probe via AFM. Bioavailability and bioactivity tests on the fiber surface demonstrate that the meshes promote adhesion and migration of fibroblasts in comparison to pure PLGA meshes. The one-step production of PLGA-based fibers could be exploited to electrospin into living cell culture without indication of toxic adverse effects on cell proliferation. This might be useful for production of cell-loaded scaffolds or biohybrid materials.
Trvalý link: http://hdl.handle.net/11104/0006401