Characterization of Lymphocyte Subsets in Patients with Common Variable Immunodeficiency Reveals Subsets of Naive Human B Cells Marked by CD24 Expression

0355461 - BTÚ 2011 RIV US eng J - Článek v odborném periodiku
Vlková, M. - Froňková, E. - Kanderová, V. - Janda, A. - Růžičková, Šárka - Litzman, J. - Šedivá, A. - Kalina, T.
Characterization of Lymphocyte Subsets in Patients with Common Variable Immunodeficiency Reveals Subsets of Naive Human B Cells Marked by CD24 Expression.
Journal of Immunology. Roč. 185, č. 11 (2010), s. 6431-6438. ISSN 0022-1767. E-ISSN 1550-6606
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: B lymphocyte immunophenotyping * naive B cells * common variable immunodeficiency
Kód oboru RIV: EC - Imunologie
Impakt faktor: 5.745, rok: 2010

B cells from 48 patients with CVID and 49 healthy controls were analyzed by polychromatic flow cytometry to examine phenotype. We have described two groups of patients with normal (CVID-21norm) or increased (CVID-21lo) proportions of CD27(neg)CD21(neg)CD38(neg) B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID-21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. In additon, two different subpopulations based on IgM and CD24 expression were found corresponding to follicular (FO) I and FO II cells previously described in mice. We propose that the CD27(neg)CD21(neg)CD38(neg) B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression
Trvalý link: http://hdl.handle.net/11104/0194225