The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549

0353772 - BFÚ 2011 RIV CZ eng J - Článek v odborném periodiku
Kolářová, Hana - Binó, Lucia - Pejchalová, Kateřina - Kubala, Lukáš
The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549.
Folia Biologica. Roč. 56, č. 5 (2010), s. 211-217. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA ČR(CZ) GA524/06/1197; GA ČR(CZ) GA524/08/1753
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: lung epithelial cells * reactive oxygen species * NADPH oxidase
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 0.729, rok: 2010

Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2,7-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.
Trvalý link: http://hdl.handle.net/11104/0192924