Protein surface shielding agents in protein crystallization

0353319 - ÚMCH 2011 RIV DK eng J - Článek v odborném periodiku
Hašek, Jindřich
Protein surface shielding agents in protein crystallization.
Journal of Synchrotron Radiation. Roč. 18, Part 1 (2011), s. 50-52. ISSN 0909-0495. E-ISSN 1600-5775.
[International Symposium on Diffraction Structural Biology /3./. Paris - Orsay, 25.05.2010-28.05.2010]
Grant CEP: GA AV ČR IAA500500701; GA ČR GA305/07/1073
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: protein adhesion * protein surface shielding agents * intermolecular interactions
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 2.726, rok: 2011

The molecules adhering temporarily on the surface of protein molecules change the propensity of protein molecules to deposit on the crystal surface in a definite position and orientation. The concepts of competitive adhesion modes and protein surface shielding agents acting on the surface of molecules in a non-equilibrium process of protein crystallization provide a useful platform for the control of crystallization. The desirable goal, i.e. a transient preference of a single dominating adhesion mode between protein molecules during crystallization, leads to uniform deposition of proteins in a crystal. This condition is the most important factor for diffraction quality and thus also for the accuracy of protein structure determination. The presented hypothesis is a generalization of the experimentally well proven behaviour of hydrophilic polymers on the surface of protein molecules of other compounds.
Trvalý link: http://hdl.handle.net/11104/0192598