Fiber-optic pH detection in small volumes of biosamples

0349751 - ÚFE 2011 RIV DE eng J - Článek v odborném periodiku
Kašík, Ivan - Mrázek, Jan - Martan, Tomáš - Pospíšilová, Marie - Podrazký, Ondřej - Matějec, Vlastimil - Hoyerová, Klára - Kamínek, Miroslav
Fiber-optic pH detection in small volumes of biosamples.
Analytical and Bioanalytical Chemistry. Roč. 398, č. 5 (2010), s. 1883-1889. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA MŠMT(CZ) LC06034; GA ČR(CZ) GP102/08/P639; GA AV ČR IAA600380805
Výzkumný záměr: CEZ:AV0Z20670512; CEZ:AV0Z50380511
Klíčová slova: optical fiber sensor * plants * pH
Kód oboru RIV: JB - Senzory, čidla, měření a regulace
Impakt faktor: 3.841, rok: 2010

The paper deals with the fiber-optic detection of the pH values of droplets of plant xylem exudate based on fluorescence intensity measurements. For this purpose, novel V-taper sensing probes with a minimum diameter of around 8 μm were prepared that enable the delivery of fluorescence signal from the detection site on the taper tip to the detector. The taper tips were coated with pH-sensitive HPTS and a reference Ru-phen dichloride immobilized in a xerogel layer. Using the developed V-taper sensing probes, the pH values of in vivo and in vitro samples of small volumes (6 μl) of exudate were measured.
Trvalý link: http://hdl.handle.net/11104/0189906