New in vitro reporter gene bioassays for screening of hormonal active compounds in the environment

0348168 - MBÚ 2011 RIV DE eng J - Článek v odborném periodiku
Svobodová, Kateřina - Cajthaml, Tomáš
New in vitro reporter gene bioassays for screening of hormonal active compounds in the environment.
Applied Microbiology and Biotechnology. Roč. 88, č. 4 (2010), s. 839-847. ISSN 0175-7598. E-ISSN 1432-0614
Grant CEP: GA ČR GAP503/10/0408
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: endocrine disruptors * in vitro bioassays * reporter gene assays
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.280, rok: 2010

Identification of chemicals with endocrinedisrupting activities in the past two decades has led to the need for sensitive assays for detection and monitoring of these activities in the environment. In vitro reporter gene assays represent a relatively fast and easy-to-perform method for detection of compounds that are able to bind to hormonal receptors and stimulate or silence their transactivation activity, thus interfering with the hormone signaling pathways. This paper reviews upgrades on reporter gene assays performed during the last decade
Trvalý link: http://hdl.handle.net/11104/0188766