Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples

0338379 - ÚIACH 2010 RIV DE eng J - Článek v odborném periodiku
Šťastná, Miroslava - Šlais, Karel
Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples.
Electrophoresis. Roč. 31, č. 3 (2010), s. 433-439. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA AV ČR IAAX00310701
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: carrier-free divergent flow IEF * proteins * yeast lysate
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.569, rok: 2010

Based on the principles of recently newly established electrophoretic method called divergent flow IEF (DF IEF), we have tested the DF IEF instrument operating without the use of background carrier ampholytes. In DF IEF, the proteins are separated, desalted and concentrated in one step. We have shown that during separation and focusing of sample consisting of high numbers of proteins (yeast lysate and wheat flour extract), the pH gradient of preparative divergent flow IEF (DF IEF) can be created by autofocusing of the sample components themselves.
Trvalý link: http://hdl.handle.net/11104/0182167