Počet záznamů: 1
Recovery of tubulin functions after freeze-dying in the presence of trehalose
- 1.0334042 - ÚMG 2010 RIV US eng J - Článek v odborném periodiku
Dráberová, Eduarda - Sulimenko, Vadym - Sulimenko, Tetyana - Böhm, K. J. - Dráber, Pavel
Recovery of tubulin functions after freeze-dying in the presence of trehalose.
Analytical Biochemistry. Roč. 397, č. 1 (2010), s. 67-72. ISSN 0003-2697. E-ISSN 1096-0309
Grant CEP: GA AV ČR KAN200520701; GA MŠMT LC545
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: Tubulin * Freeze-drying * Stability
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.236, rok: 2010
We describe a novel method for freeze-drying of assembly-competent tubulin in the presence of a nonreducing sugar trehalose. Even after prolonged storage at ambient temperature, rehydrated tubulin is capable of binding antimitotic drugs and assembling to microtubules that bind microtubule-associated proteins in the usual way.
Trvalý link: http://hdl.handle.net/11104/0178877
Počet záznamů: 1