Počet záznamů: 1
Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
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SYSNO ASEP 0482642 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging Tvůrce(i) Lukeš, T. (CZ)
Glatzová, Daniela (UFCH-W)
Kvíčalová, Zuzana (UFCH-W)
Levet, F. (FR)
Benda, Aleš (UFCH-W) RID, ORCID
Letschert, S. (DE)
Sauer, M. (DE)
Brdička, Tomáš (UMG-J) RID
Lasser, T. (FR)
Cebecauer, Marek (UFCH-W) RID, ORCID, SAIČíslo článku 1731 Zdroj.dok. Nature Communications. - : Nature Publishing Group
Roč. 8, č. 1 (2017)Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova quantifying protein densities ; membranes ; single-molecule localization microscopy Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA15-06989S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 ; UMG-J - RVO:68378050 UT WOS 000416229300017 EID SCOPUS 85035071940 DOI 10.1038/s41467-017-01857-x Anotace Quantitative approaches for characterizing molecular organization of cell membrane molecules under physiological and pathological conditions profit from recently developed super-resolution imaging techniques. Current tools employ statistical algorithms to determine clusters of molecules based on single-molecule localization microscopy (SMLM) data. These approaches are limited by the ability of SMLM techniques to identify and localize molecules in densely populated areas and experimental conditions of sample preparation and image acquisition. We have developed a robust, model-free, quantitative clustering analysis to determine the distribution of membrane molecules that excels in densely labeled areas and is tolerant to various experimental conditions, i.e. multiple-blinking or high blinking rates. The method is based on a TIRF microscope followed by a super-resolution optical fluctuation imaging (SOFI) analysis. The effectiveness and robustness of the method is validated using simulated and experimental data investigating nanoscale distribution of CD4 glycoprotein mutants in the plasma membrane of T cells. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2018
Počet záznamů: 1