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The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate
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SYSNO ASEP 0475665 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate Tvůrce(i) Sjöholm, C. (SE)
Bergstrand, J. (SE)
Nilsson, T. (SE)
Šachl, Radek (UFCH-W) RID, ORCID
von Ballmoos, Ch. (CH)
Widengren, J. (SE)
Brzezinski, P. (SE)Číslo článku 2926 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, č. 1 (2017)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova biological energy-conversion ; cytochrome-c-oxidase ; membrane-surface ; rhodobacter-sphaeroides Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000402879200011 EID SCOPUS 85020432705 DOI https://doi.org/10.1038/s41598-017-02836-4 Anotace We have investigated the effect of lipid composition on interactions between cytochrome bo(3) and ATP-synthase, and the ATP-synthesis activity driven by proton pumping. The two proteins were labeled by fluorescent probes and co-reconstituted in large (d congruent to 100 nm) or giant (d congruent to 10 mu m) unilamellar lipid vesicles. Interactions were investigated using fluorescence correlation/cross-correlation spectroscopy and the activity was determined by measuring ATP production, driven by electron-proton transfer, as a function of time. We found that conditions that promoted direct interactions between the two proteins in the membrane (higher fraction DOPC lipids or labeling by hydrophobic molecules) correlated with an increased activity. These data indicate that the ATP-synthesis rate increases with decreasing distance between cytochrome bo3 and the ATP-synthase, and involves proton transfer along the membrane surface. The maximum distance for lateral proton transfer along the surface was found to be similar to 80 nm. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2018
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