High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli

0369717 - MBÚ 2012 RIV US eng J - Článek v odborném periodiku
Rozbeský, Daniel - Kavan, Daniel - Chmelík, Josef - Novák, Petr - Vaněk, Ondřej - Bezouška, Karel
High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli.
Protein Expression and Purification. Roč. 77, č. 2 (2011), s. 178-184. ISSN 1046-5928. E-ISSN 1096-0279
Grant CEP: GA ČR GA303/09/0477; GA ČR GD305/09/H008; GA ČR GAP207/10/1040; GA AV ČR IAA500200620; GA MŠMT LC07017; GA MŠMT LC545; GA MŠMT 1M0505
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Natural killer cell * NKR-P1C receptor * NK1.1 antigen
Kód oboru RIV: EC - Imunologie
Impakt faktor: 1.587, rok: 2011

Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain
Trvalý link: http://hdl.handle.net/11104/0203718