An invertebrate [hydroxyproline]-modified neuropeptide: further evidence for a close evolutionary relationship between insect adipokinetic hormone and mammalian gonadotropin hormone family

0368706 - BC 2012 RIV US eng J - Článek v odborném periodiku
Gäde, G. - Šimek, Petr - Marco, H. G.
An invertebrate [hydroxyproline]-modified neuropeptide: further evidence for a close evolutionary relationship between insect adipokinetic hormone and mammalian gonadotropin hormone family.
Biochemical and Biophysical Research Communications. Roč. 414, č. 3 (2011), s. 592-597. ISSN 0006-291X. E-ISSN 1090-2104
Grant CEP: GA ČR GAP206/10/2401
Grant ostatní: National Research Foundation, Pretoria(ZA) FA2007021300002; National Research Foundation, Pretoria(ZA) IFR 2008071500048
Výzkumný záměr: CEZ:AV0Z50070508
Klíčová slova: Insects * stink bug * Nezara viridula
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 2.484, rok: 2011

An octapeptide of the adipokinetic hormone (AKH) peptide family is identified in corpora cardiaca of the stink bug, Nezara viridula. This is the second AKH in N. viridula and it has a hydroxyproline residue at position 6, whereas the major AKH (known as Panbo-RPCH) has Pro as the sixth amino acid residue. Various extraction procedures were followed to ascertain whether the hydroxylation is an artefact of extraction, or whether it is due to a true post-translational modification at the prohormone level. The proline hydroxylation is unique for invertebrate neuropeptides, while it has been described in the vertebrate gonadotropin-releasing hormone (GnRH). The current finding is another piece of evidence that AKH and GnRH form a peptide superfamily and are closely related evolutionarily. Biologically, [Hyp6]-Panbo-RPCH is active in vivo as an AKH, causing hyperlipaemia in the stink bug at low doses, indicating that it is an endogenous, mature and functional hormone in this insect species.
Trvalý link: http://hdl.handle.net/11104/0202978