Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

0364524 - ÚŽFG 2012 RIV US eng J - Článek v odborném periodiku
Vodičková Kepková, Kateřina - Vodička, Petr - Toralová, Tereza - Lopatářová, M. - Čech, S. - Doležel, R. - Havlíček, V. - Besenfelder, U. - Kuzmany, H. - Sirard, M. A. - Laurinčik, J. - Kaňka, Jiří
Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition.
Theriogenelogy. Roč. 75, č. 9 (2011), s. 1582-1595. ISSN 0093-691X. E-ISSN 1879-3231
Grant CEP: GA MŠMT 1M0538; GA ČR GA523/09/1035; GA ČR(CZ) GD204/09/H084
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: OOCYTE MATURATION MEDIA * MESSENGER-RNA LEVELS * EARLY EMBRYOGENESIS
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.963, rok: 2011

Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine oocyte and preimplantation embryo-specific cDNAs (BlueChip, Universite Laval, Quebec). The analysis revealed changes in the level of 134 transcripts between in vitro derived (cultured in COOK BVC/BVB media) and in vivo derived 4-cell stage embryos and 97 transcripts were differentially expressed between 8-cell stage in vitro and in vivo embryos. The expression profiles of 7 selected transcripts (BUB3, CUL1, FBL, NOLC1, PCAF, GABPA and CNOT4) were studied in detail. We have identified a switch from Cullin 1-like transcript variant 1 to Cullin 1 transcript variant 3 (UniGene IDs BT.36789 and BT.6490, respectively) expressions around the time of bovine major gene activation (8-cell stage).
Trvalý link: http://hdl.handle.net/11104/0199991