Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells

0362824 - MBÚ 2012 RIV US eng J - Článek v odborném periodiku
Kodedová, M. - Sigler, Karel - Lemire, B. D. - Gášková, Dana
Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells.
Biotechniques. Roč. 50, č. 1 (2011), 58-63. ISSN 0736-6205. E-ISSN 1940-9818
Grant CEP: GA MŠMT 1M0570
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: fluorescent dye diS-C(3)(3) * membrane potential * surface-active drug
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.669, rok: 2011

New antifungal agents are needed to treat life-threatening fungal infections, particularly with the development of resistance. Surface-active antifungals have the advantages of minimizing host toxicity and the emergence of drug resistance. We have developed a time-dependent drug exposure assay that allows us to rapidly investigate the mechanism of surface-active antifungal drug action. The assay uses a multidrug pump-deficient strain of Saccharomyces cerevisiae and the potentiometric dye 3,3-dipropylthiacarbocyanine iodide [diS-C3(3)] and can assess whether cells are depolarized, hyperpolarized, or permeabilized by drug exposure
Trvalý link: http://hdl.handle.net/11104/0199022