Haplotype analysis of the interleukin-18 gene in Czech patients with allergic disorders

0360883 - ÚŽFG 2012 RIV US eng J - Článek v odborném periodiku
Izakovicova Holla, L. - Hrdličková, B. - Schüller, M. - Bučková, Dana - Kindlova, D. - Izakovic, V. - Vasku, A.
Haplotype analysis of the interleukin-18 gene in Czech patients with allergic disorders.
Human Immunology. Roč. 71, č. 6 (2010), s. 592-597. ISSN 0198-8859. E-ISSN 1879-1166
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: allergic diseases * asthma * IL-18
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.872, rok: 2010

The interleukin-18 (IL-18) gene on chromosome 11q22 has been suggested as a susceptibility factor for allergies. To test for a possible role of IL-18 polymorphisms in Czech population, case-control study including 958 subjects (633 allergic patients and 325 healthy controls) was performed. An allele-specific polymerase chain reaction was used to analyze variants at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the promoter region together with the polymerase chain reaction-restriction fragment length polymorphism method for the detection of polymorphism at position -140 C/G (previously -133 C/G, rs360721) in intron 1 of the IL-18 gene. The -1297 C/T (rs360719) polymorphism was genotyped by real-time-polymerase chain reaction, using a predevelopment TaqMan allele discrimination assay. There were no significant differences in distribution of alleles or genotypes in any of four single nucleotide polymorphisms in the IL-18 gene between controls and patients.
Trvalý link: http://hdl.handle.net/11104/0198332