Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni

0358458 - BC 2012 RIV US eng J - Článek v odborném periodiku
Feng, Y. - Yang, W. - Ryan, U. M. - Zhang, L. - Kváč, Martin - Koudela, Břetislav - Modrý, David - Li, N. - Fayer, R. - Xiao, L.
Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni.
Journal of Clinical Microbiology. Roč. 49, č. 1 (2011), s. 34-41. ISSN 0095-1137. E-ISSN 1098-660X
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: ASTERN UNITED-STATES * RIBOSOMAL-RNA GENE * PHYLOGENETIC ANALYSIS * MOLECULAR ANALYSIS * NATURAL INFECTION * DAIRY-CATTLE * PREVALENCE * GENOTYPES * HUMANS * KENYA
Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
Impakt faktor: 4.153, rok: 2011

Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni.
Trvalý link: http://hdl.handle.net/11104/0196498