Cancer Drug-Resistance and a Look at Specific Proteins: Rho GDP-Dissociation Inhibitor 2, Y-Box Binding Protein 1, and HSP70/90 Organizing Protein in Proteomics Clinical Application

0356672 - ÚŽFG 2011 RIV US eng J - Článek v odborném periodiku
Skalníková, Helena - Martinková, Jiřina - Hrabáková, Rita - Halada, Petr - Dziechciarková, M. - Hajdúch, M. - Gadher, S. J. - Hammar, A. - Enetoft, D. - Ekefjard, A. - Forsstrom-Olsson, O. - Kovářová, Hana
Cancer Drug-Resistance and a Look at Specific Proteins: Rho GDP-Dissociation Inhibitor 2, Y-Box Binding Protein 1, and HSP70/90 Organizing Protein in Proteomics Clinical Application.
Journal of Proteome Research. Roč. 10, č. 2 (2011), s. 404-415. ISSN 1535-3893. E-ISSN 1535-3907
Grant CEP: GA MŠMT LC07017; GA ČR GA301/08/1649
Grant ostatní: Operational Program Research and Development for Innovations(CZ) CZ.1.05/2.1.00/01.0030
Výzkumný záměr: CEZ:AV0Z50450515; CEZ:AV0Z50200510
Klíčová slova: cancer * anti-cancer drugs * drug resistance
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.113, rok: 2011

Resistance to anti-cancer drugs is a well recognized problem and very often it is responsible for failure of the cancer treatment. In this study, the proteome alterations associated with the development of acquired resistance to cyclin-depedent kinases inhibitor bohemine, a promising anti-cancer drug, were analyzed with the primary aim of identifying potential targets of resistance within the cell that could pave a way to selective elimination of specific resistant cell types. A model of parental susceptible CEM T-lymphoblastic leukemia cells and its resistant counterpart CEM-BOH was used and advanced 2-D liquid chromatography was applied to fractionate cellular proteins. Differentially expressed identified proteins were further verified using immunoblotting and immunohistochemistry. Our study has revealed that Rho GDP-dissociation inhibitor 2, Y-box binding protein 1, and the HSP70/90 organizing protein have a critical role to play in resistance to cyclin-depedent kinases inhibitor. The results indicated not only that quantitative protein changes play an important role in drug-resistance, but also that there are various other parameters such as truncation, post-translational modification(s), and subcellular localization of selected proteins. Furthermore, these proteins were validated for their roles in drug resistance using different cell lines resistant to diverse representatives of anti-cancer drugs such as vincristine and daunorubicin
Trvalý link: http://hdl.handle.net/11104/0195132