Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation

0354392 - MBÚ 2011 RIV US eng J - Článek v odborném periodiku
Nemoto, N. - Singh, Ch. R. - Udagawa, T. - Wang, S. - Thorson, E. - Winter, Z. - Ohira, T. - Li, M. - Valášek, Leoš - Brown, S. J. - Asano, K.
Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation.
Journal of Biological Chemistry. Roč. 285, č. 42 (2010), s. 32200-32212. ISSN 0021-9258. E-ISSN 1083-351X
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: START CODON SELECTION * SACCHAROMYCES-CEREVISIAE * IN-VIVO
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 5.328, rok: 2010

The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3(eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the mRNA entry channel of the 40S subunit. We have identified substitutions in the conserved Lys-Glu-Arg-Arg (KERR) motif and in residues of the nearby box6 element of the a/Tif32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes indicating defects in scanning and start codon recognition. The normally dispensable CTD of j/Hcr1 is required for its binding to a/Tif32 and to mitigate the growth defects of these a/Tif32 mutants, indicating physical and functional interactions between these two domains
Trvalý link: http://hdl.handle.net/11104/0193403