Immobilization of CGTases from Bacillus circulans and Bacillus pseudalcaliphilus on aminosilane and PEI modified magnetic nanoparticles and modified silica particles

0351749 - ÚVGZ 2011 RIV BG eng J - Článek v odborném periodiku
Ivanova, V. - Tonkova, A. - Atanassova, N. - Šafaříková, Miroslava - Hristov, J.
Immobilization of CGTases from Bacillus circulans and Bacillus pseudalcaliphilus on aminosilane and PEI modified magnetic nanoparticles and modified silica particles.
International Review of Chemical Engineering. Roč. 2, č. 2 (2010), s. 278-288. ISSN 2035-1755.
[1st Conference on Chemical Engineering and Advanced Materials. Naples, 23.11.2009-08.12.2009]
Grant CEP: GA MPO(CZ) 2A-1TP1/094
Výzkumný záměr: CEZ:AV0Z60870520
Klíčová slova: cyclodextrin glucanotransferase * immobilization * magnetic particles
Kód oboru RIV: CE - Biochemie

The extracellular enzymes cyclodextrin glucanotransferases produces by Bacillus circulans ATCC 21783, Bacillus pseudoalcaliphilus strain 8SB and Bacillus pseudoalcaliphilus strain 20RF were immobilized by covalent linking via glutaraldehyde to silanized magnetic nanoparticles treated with PEI, silanized magnetite and magnetic nanoprarticles, and commercionally functionalized silica nano- and microparticles in order to study the immobilization capacities of the carriers, the activity recovery and some biochemical characteristics of the enzymes after immobilization. The immobilized enzymes showed a high operational stability after twelve re-uses.
Trvalý link: http://hdl.handle.net/11104/0191432