Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants

0350379 - ÚEB 2011 RIV NL eng J - Článek v odborném periodiku
Dwivedi, S. - Vaňková, Radomíra - Motyka, Václav - Herrera, C. - Žižková, Eva - Auer, C.
Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants.
Plant Growth Regulation. Roč. 61, č. 3 (2010), s. 231-242. ISSN 0167-6903. E-ISSN 1573-5087
Grant CEP: GA AV ČR IAA600380701; GA MŠMT(CZ) LC06034; GA MŠMT ME 505
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Cytokinin metabolism * N-Glucosyltransferase * GRAM domain
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 1.630, rok: 2010

Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants.
Trvalý link: http://hdl.handle.net/11104/0190396