Peroxidative damage of mitochondrial respiration is substrate-dependent

0338968 - FGÚ 2010 RIV CZ eng J - Článek v odborném periodiku
Endlicher, R. - Křiváková, P. - Rauchová, Hana - Nůsková, Hana - Červinková, Z. - Drahota, Zdeněk
Peroxidative damage of mitochondrial respiration is substrate-dependent.
Physiological Research. Roč. 58, č. 5 (2009), s. 685-692. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA ČR(CZ) GA303/06/1261
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: mitochondrial enzymes * peroxidative damage * tert-butyl hydroperoxide
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.430, rok: 2009

The concentration-dependence of tert-butyl hydroperoxide (BHP) inhibitory effect on oxygen consumption in isolated rat liver mitochondria was measured in the presence of various respiratory substrates. Strong inhibitory effect at low concentrations of BHP (15-30 µM) was found for oxoglutarate and palmitoyl carnitine oxidation. Our data indicate that beside peroxidative damage of complex I also various mitochondrial NADH-dependent dehydrogenases are inhibited but to a different extent and with different kinetics. Our data also show that succinate could be an important nutritional substrate protecting hepatocytes during peroxidative damage
Trvalý link: http://hdl.handle.net/11104/0182609