Biocatalytic application of nitrilases from Fusarium solani O1 and Aspergillus niger K10

0338588 - MBÚ 2010 RIV NL eng J - Článek v odborném periodiku
Winkler, M. - Kaplan, Ondřej - Vejvoda, Vojtěch - Klempier, N. - Martínková, Ludmila
Biocatalytic application of nitrilases from Fusarium solani O1 and Aspergillus niger K10.
Journal of Molecular Catalysis B-Enzymatic. Roč. 59, č. 4 (2009), s. 243-247. ISSN 1381-1177
Grant CEP: GA AV ČR IAA500200708; GA MŠMT(CZ) LC06010
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Amino nitriles * Nitrilase * Substrate specificity
Kód oboru RIV: CE - Biochemie
Impakt faktor: 2.400, rok: 2009

The nitrilases from Fusarium solani O1 and Aspergillus niger K10 showed a broad substrate specificity for carbocyclic and nonaromatic heterocyclic amino nitriles, the preferred substrates being five-membered g-amino nitrile , six-membered g-amino nitriles , and , pyrrolidine-3-carbonitriles and as well as piperidine-4-carbonitriles 14a and 15a. Both enzymes showed a strong diastereopreference for cis- vs. trans-g-amino nitriles. The electronic and steric effects of N-protecting groups affected the reactivity of the nitriles. Amides as by-products of the nitrilase-catalyzed reaction were produced from heterocyclic amino nitriles, 14a and 15a by the A. niger enzyme but only from nitrile by the F. solani enzyme.
Trvalý link: http://hdl.handle.net/11104/0182320