Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules

0337286 - ÚEB 2010 RIV US eng J - Článek v odborném periodiku
Andreeva, Z. - Ho, A. Y. Y. - Barthet, M. M. - Potocký, Martin - Bezvoda, R. - Žárský, Viktor - Marc, J.
Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules.
Functional Plant Biology. Roč. 36, č. 7 (2009), s. 600-612. ISSN 1445-4408. E-ISSN 1445-4416
Grant CEP: GA AV ČR IAA601110916
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Allium * Arabidopsis * F-actin-microtubule interactions
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.678, rok: 2009

Phospholipase D (PLD) is a key enzyme in signal transduction-mediating plant responses to various environmental stresses including drought and salinity. Isotype PLD delta interacts with the microtubule cytoskeleton, although it is unclear if, or how, each of the 12 PLD isotypes in Arabidopsis may be involved mechanistically. We employed RNA interference in epidermal cells of Allium porrum L. (leek) leaves, in which the developmental reorientation of cortical microtubule arrays to a longitudinal direction is highly sensitive to experimental manipulation. Using particle bombardment and transient transformation with synthetic siRNAs targeting AtPLD alpha, beta, gamma, delta, epsilon and zeta, we examined the effect of 'cross-target' silencing orthologous A. porrum genes on microtubule reorientation dynamics during cell elongation. Co-transformation of individual siRNAs together with a GFP-MBD microtubule-reporter gene revealed that siRNAs targeting AtPLD delta promoted, whereas siRNAs targeting AtPLD.
Trvalý link: http://hdl.handle.net/11104/0181314